Medical Technology Board Examination Review Notes on Virology

Share:


A mature virus particle containing a nucleic acid core surrounded by a protein coat, with or without envelope, is called:

VIRION
Viruses are characterized by the presence of:
DNA AND RNA
What is the largest DNA virus?
POXVIRUS
Which is the smallest RNA virus?
ENTEROVIRUS
What is the first step in the replication cycle of a virus?
ATTACHMENT AND PENETRATION
The virus capsid morphology is:
HELICAL OR ICOSAHEDRAL
From what part of the virus is the envelop acquired?
NUCLEAR OR CYTOPLASMIC MEMBRANE
Where is the site of virion assembly?
NUCLEUS OR CYTOPLASM
Which of the following is a DNA virus?
HERPESVIRIDAE
Which of the following is an RNA virus?
PSEUDOMYXOVIRIDAE
Specimens collected for virus isolation should be kept at:
4C


At what temperature should clinical specimens suspected of containing viruses kept for transport that takes days?
-70C
In what family of viruses, after primary infection, does an individual become latently infected and then the infection can be reactivated?
HERPESVIRIDAE
Cytomegalovirus isolation is best accomplished using:
HUMAN EMBRYONIC FIBROBLASTS
Which of the following viruses causes acute central nervous system disease in humans and animals?
RABIES
What specimen/s should be collected from a patient with suspected enteroviral meningitis?
STOOL, THROAT SWAB, CSF
Influenza A viruses can be detected in cell cultures by:
HEMADSORPTION ASSAY
The best host systems for influenza virus isolation are:
MONKEY KIDNEY CELLS AND EMBRYONATED HEN’S EGGS
What common antigen is cross-reactive in all human adenoviruses?
HEXON
The following characteristics are similar in both influenza and parainfluenza viruses
HELICAL SYMMETRY, LIPID ENVELOPE, BUDDNG AND CYTOPLASMIC MEMBRANE
Myxoviridae virus envelope contains both:
NEURAMINIDASE AND HEMAGGLUTININ
Eosinophilic cytoplasmic inclusions are elemtary bodies of the:
POXVIRUS
German measles is caused by:
RUBELLA VIRUS
What is an alastrim infection?
MILD FORM OF SMALLPOX
Which of the following procedure is routinely used for the detection of Hepatitis B virus (HBV) in blood donors?
SOLID-PHASE RIA AND ELISA
Which of the following laboratory procedures is the most rapid method for diagnosis of a virus infection?
DIRECT ELECTRON MICROSCOPY
What specimens should be collected from a patient with suspected influenza?
THROAT SWAB
What virus group contains a double stranded, segmented RNA genome?
REOVIRUS
Enterovirus can be differentiated from rhinoviruses by:
ACID RESISTANCE
What virus is the most frequent cause of gastroenteritis in children during the winter months?
ROTAVIRUS
All of the following groups of viruses are resistant to ether treatment except:
HERPESVIRUSES
The most common method for rapid electron microscopy examination is:
NEGATIVE STAINING
Various virus groups can be differentiated in negative stained specimens by their:
MORPHOLOGY
PARASITOLOGY QUESTIONS
The motile, reproducing stage, feeding stage of the Protozoa is:
TROPHOZOITE
Which of the following amoeba has chromatoid bodies in the cyst stage?
ENTAMOEBA
Amebiasis is caused by
E. HISTOLYTICA
Which of the following organs of the body is most often involved in extraintestinal amebiasis?
LIVER
Which of the following is characteristic of true amebiasis?
- PERIPHERAL CHROMATIN ON THE NUCLEAR BODY
- CHROMATIN BODIES IN THE CYST
- INCLUDE PATHOGENS AND NONPATHOGENS
Which of the following is a true ameba?
E. HARTMANNI
Amebae that inhabits the gastrointestinal tract of man are nonmotile, nonfeeding, and infective during which stage?
CYST
Mature cysts of E. polecki have how many nuclei?
1
Which of the following has cysts with chromatoid bodies that have two pointed ends or that can be round, triangular or oval?
E. COLI
Trophozoites of Entameoba histolytica have the following characteristics:
- SMALLL, DELICATE NUCLEAR CHROMATIN
- FINE, EVEN, PERIPHERAL CHROMATIN
- PROGRESSIVE MOTILITY WITH HYALINE, FINGER-LIKE PSEUDOPODS
Which of these trophozoites, when acting as a pathogen, is likely to ingest red blood cells of the host?
E. HISTOLYTICA
The point of differentiation between Entamoeba histolytica and Entamoeba hartmanni is?
SIZE
The mature cyst of Entamoeba histolytica has how many nuclei?
FOUR
Failure to find bacteria in purulent spinal fluid should alert one to find the possibility of an infection with?
AMEBAE
Which trophozoite is distinguished by the possession of achromatic granules surrounding its karyosomal chromatin?
I. BUTSCHLII
Which of the following would have double-walled, wrinkled cyst form?
A. CASTELLANII
A cyst that possesses a single nucleus and a large glycogen vacuole that stains deeply with iodine belong to:
I. BUTSCHLII
Some amebae have spiny, hyaline extensions called:
ACANTHOPODS
Which are the best staining procedures for species of Naegleria and Acanthamoeba?
H&E  AND WRIGHT’S STAIN
Amebae inhabiting the central nervous system enter the body through?
NASAL MUCOSA
In which specimen are Acanthamoeba and Naegleria usually found?
CEREBROSPINAL FLUID
Naegleria has which of the following characteristics
- FOUND IN THE BRAIN
- TROPHOZOITE CAN ASSUME A LIMAX FORM
- TROPHOZOITE CAN BECOME AN AMEBA FLAGELLATE
The trophozoite whose karyosomal chromatin appears as a rosette of 4-6 granules is identified as:
N. FOWLERI
Which of the following are often mistaken for cysts of amebae?
BLASTOCYSTIS HOMINIS
Intestinal flagellates are usually which shape in the trophozoite stage?
PEAR-SHAPED
Which of the following is pathognomonic for G. lamblia and the stage it is found in?
VENTRAL SUCKING DISK- TROPHOZOITE
Which of the following protozoa have an undulating membrane?
TRICHOMONAS, TRYPANOSOMA
Infections with D. fragilis can show which of the following symptoms?
DIARRHEA
ABDOMINAL DISCOMFORT WITHOUT DIARRHEA
ASYMPTOMATIC
What does D. fragilis have in common with T. vaginalis?
NEITHER HAS CYST FOR
Which flagellate can be a pathogen of small intestines?
GIARDIA
Which intestinal flagellate trophozoite has a sucking disk, 2 nucleo, 8 flagella and an axostyle?
G. LAMBLIA
Red and white blood cells in stool specimens are characteristic of?
BACILLARY DYSENTERY
A pear-shaped flagellate with jerky motility that is found in a urine specimen is identified as?
T. VAGINALIS
Eighty percent of the trophozoites of D. fragilis have?
2 NUCLEI
The only bilaterally symmetrical protozoan is?
GIARDIA
Whoch of the following is the intracellular form of blood and tissue flagellates?
LEISHMANIAL
Which of the following is the cause of African sleeping sickness?
TRYPANOSOMA
Which of the following is the vector of African sleeping sickness?
TSETSE FLY
Which species of Trypanosoma is the cause of Chagas disease?
CRUZI
Which of the following is characteristic of the trypanosome form of Trypanosoma cruzi?
PROMINENT KINETOPLAST
C-SHAPED
THICK ORGANISM
Which specimen would be suitable for a demonstration of the trypanosomes of sleeping sickness?
BLOOD
FLUID FROM LYMPH NODE
CSF
The extracellular form of Trypanosoma is a slender organism characterized by an undulating membrane and a free flagellum that arises:
POSTERIORLY FROM THE KINETOPLAST
Which of the following is found within the reticuloendothelial cells?
L. DONOVANI
H. CAPSULATUM
T. GONDII
How is the Trypanosoma cruzi transmitted?
BITE OF REDUVIID BUG
A chagoma is a lesion deen in infections with:
TRYPANOSOME CRUZI
Which organism is the cause of kala-azar?
L. DONOVANI
In the laboratory diagnosis of L. donovani, which is the preferred specimen in which to find Leihman-Donovan bodies?
BONE MARROW
Which of the following is the only ciliate that is pathogenic in humans?
B COLI
A very large cyst whose double wall encloses a ciliated organism with one visible nuclei would be:
ACQUIRED BY ACCIDENT BY MAN, SINCE IT CUSTOMARILY INFECTS SWINE
Which of the following structures are used for the motility of Balantidium coli?
CILIA
Which nucleus in the trophozoite of Balantidium coli is the reproductive one?
MICRONUCLEUS
Conjugation of trophozoites of Balantidium coli never occurs between:
SAME SIZE ORGANISMS
The definitive host (vector) to Plasmodium is the:
FEMALE ANOPHELES MOSQUITO
Which of the malarial organisms presents as pale, very ameboid ring trophozoites, infecting a large pale red blood cell with dots of hemoglobin?
P. VIVAX
Which of the malarial organisms preferentially invades reticulocytes?
P. VIVAX
The malarial organism whose schizont resembles a “fruit pie” in which the merozoite form a rosette around the malarial pigment is:
MALARIAE
The malarial organism characteristically has a band form trophozoite stretching across the red blood cell?
P. MALARIAE
The gametocyte of Plasmodium falciparum can be differentiated from that of other malarial species by?
SHAPE
In which type of malaria can Ziemann’s stippling be found?
MALARIAE
In which type of malaria can Maurer’s dots be found?
P. FALCIPARUM
Which malarial organism has large, coarse, red dots with a large, pale red blood cell with fimbriated edges?
P. OVALE
The sexual reproduction cycle in Plasmodiu, and Coccidia is referred to as:
SPOROGONY
What is the infective stage of the malarial parasite to the vector?
GAMETOCYTE
What is the infective stage of the malarial parasite to humans?
SPOROZOITES
In which type of malaria is there synchronized rupture of the red blood cells every 72 hours?
MALARIAE
In falciparum malaria, there may be a sudden massive intravascular hemolysis producing hemoglobinuria. This is called:
BLACKWATER FEVER
Which hemoglobin is incompatible with malarial parasite survival?
HB-SS
What is the name of the laboratory test that allows laboratory bred reduviid bugs to feed on patients suspected of having Chagas’ disease?
XENODIAGNOSIS

Human are infected with Babesia by:
BITE OF TICKS
BLOOD TRANSFUSION
If immature oocysts of Isospora belli are found in stool specimens from infected humans, what should be done with the specimen for identification?
LEAVE AT ROOM TEMPERATURE
What stage of Isospora is infective to humans?
OOCYSTS
Isospora belli immature oocysts contain/s:
SPOROBLAST
Infective oocysts of Isospora belli contain:
SPOROZOITES
The demonstration of retinochoroiditis and cerebral calcifications in a newborn would result in which of these laboratory requests:
TITER OF TOXOPLASMA ANTIBODIES
What is both the definitive and intermediate host of Toxoplasma gondii:
CAT
What is the appearance of Toxoplasma gondii in tissue fluids of man?
CRESCENT
What constitutes a positive result in the Sabin-Feldman dye test?
TOXOPLASMA LOSES ITS AFFINITY FOR METHYLENE BLUE DYE
Which of the following tests is used for the detection of Cryptosporidium?
SHEATHER’S SUGAR FLOATATION
Which of the following parasites poses a particular hazard for immunodeficient or immunosuppressed individuals?
CRYPTOSPORIDIUM, GIARDIA, STRONGYLOIDES
Which of the followi0ng parasites is associated with AIDS?
CRYPTOSPORIDIUM
Which operculated ovum contains a miracidium and can appear in sputum, often accompanied by blood and Charcot-Leyden crystals?
PARAGONIMUS WESTERMANI
Which of the schistosomes may be recovered in rectal biopsy?
SCHISTOSOMA MANSONI AND SCHISTOSOMA JAPONICUM
The scientific name of the head of a tapeworm is:
SCOLEX
Which of the following worms have an oral and ventral shaped sucker in the adult stage?
TREMATODES
Which of the following are hermaphroditic?
FLUKES AND ROUNDWORMS
Which of the following is the first intermediate host of the flukes?
SNAIL
The common names for the schostosomes is:
BLOOD FLUKES
What is the common name for Clonorchis sinensis?
CHINESE LIVER FLUKE
What is a schistosomule?
CERCARIAE MINUS TAIL
The beef tapeworm is the common name for?
T. SAGINATA
Which tapeworm proglottid makes its way across the fecal specimen by doubling movements, and is seen under the microscope to have numerous regular uterine branches (more that 15) resembling those of a tree?
T. SAGINATA
Which of the following has an unarmed scolex?
BEEF TAPEWORM
Which species of Taenia has 7 to 12 uterine branches?
T. SOLIUM
A hexacanth embryo enclosed in a radially striated shell belongs to the genus
TAENIA
Which tapeworm infection has to be treated with great care so that man does not acquire the larval infection:
TAENIASIS SOLIUM
A bile stained egg that is 75 microns at its greatest diameter and contains a hexacanth embryo that lacks polar knobs or filaments is that of:
H. DIMINUTA
The “dwarf” tapeworm is another name for:
H. NANA
The eggs of Echinococcus granulosus are found in the feces of:
DOGS
Which of the following is found in the intermediate host of Echonococcus granulosus?
HYDATID CYST
Hydatid cyst infection of man is due to a larva of the tapeworm:
E. GRANULOSUS
The first intermediate host of Diphylloborhrium latum is:
COPEPOD
The second intermediate host of Diphylloborhrium latum is:
FRESHWATER FISH
What is the infective stage of the broad fish tapeworm to humans?
PLEROCERCOID
The eggs of this tapeworm are developed and have an operculum at one end and a small abopercular knob at the other end. The tapeworm is:
D. LATUM
In some individuals, particularly those of Scandinavian extraction, this worm can cause megaloblastic anemia.
D. LATUM
Which of the following worms have separate sexes
BLOOD FLUKES
The male roundworm is differentiated from the female roundworm by its:
DORSALLY CURVED POSTERIOR
The best way to demonstrate a pinworm infection is by which of the following techniques?
CELLOPHANE TAPE PREPARATION
The gravid female of Enterobius vermicularis deposits her embryonated eggs:
ON THE PERIANAL SKIN
Eggs that average 60 microns in lenghth, are oval with one flattened side, and contain a motile larva, are those of:
E. VERMICULARIS
Eggs that are bile-stained and have clear polar plugs belong to the:
WHIPWORM
Which of the following nematodes does NOT have a free-living state:
E. VERMICULARIS
Whicj stage of Trichuris trichiura is infective to humans?
EMBRYONATED EGG
Where does the larva of Ascaris lumbricoides go after it hatches In the small intestines?
MIGRATES THROUGH THE BLOOD, LIVER, LUNGS, PHARYNX, AND THEN BACK TO THE SMALL INTESTINES
If an Ascaris egg lacks its bile-stained mamillated coat, we refer to the egg as:
DECORTICATED
Trichuris trichiura, hookworm and ____ form the “unholy three” of roundworms:
A. LUMBRICOIDES
Which of the following organisms caused infections where the larvae migrate into the lymphatics and blood, lung, alveoli, bronchiolus, pharynx, and then the small intestines:
N. AMERICANUS,
 A. DUODENALE
S. STERCOLARIS
Which of the following is characteristic of the eggs of hookworms?
AN EMBRYO I0N THE TWO-TO-EIGHT CELL STAGE OF CLEAVAGE
OVA, THIN SHELL
CLEAR SPACE BETWEEN SHELL AND EMBRYO
The helminth that induces a hypochromic microcytic anemia in its host is the:
HOOKWORM
The rhabtidiform larva of the hookworm has a:
LONG BUCCAL CAVITY EQUAL TO THE WIDTH OF THE BODY
Which of the following larval worms enters the host by penetration of the skin?
S. STERCORALIS; A. DUODENALE; N. AMERICANUS
A roundworm that ingabits the small intestine and usually is demonstrated as rhabditiform larvae in the fecal specimen is the:
THREADWORM
Muscle biopsy is a diagnostic technique employed to detect:
TRICHINOSIS
Which of the following roundworms give birth to her young:
WUCHERERIA; MANSONELLA; BRUGIA
A viviparous female nematode:
PRODICE LIVING LARVAE
One of the differential characteristics of the microfilariae is the presence or absence of:
A SHEATH
Adults of filarial worms live in:
LYMPHATICS AND SUBCUTANEOUS TISSUE
One of the pathological findings in infestations of Wuchereria is:
ELEPHANTIASIS
The intermediate host of the guinea worm is the:
COPEPOD
Dirofilaria immitis is the:
DOG HEARTWORM
Dracunculus infections cause ulcers that:
ON CONTACT WITH WATER, RELEASE LARVAE
Which of the following microfilariae does NOT have a sheath
MANSONELLA
Which of the following is the eye worm
LOA
Cutaneous larva migrans or creeping eruption is caused by:
FILARIFORM LARVAE OF DOG HOOKWORM
Which of the following causes a chronic cough, pulmonary and artery obstruction but no invasion of the heart in humans?
DIROFILARIA IMMITIS
Which of the following is caused by the ingestion of snails by humans?
RAT LUNGWORM
Cercarial dermatitis, commonly known as swimmer’s itch, is caused by:
CERCARIAE OF SCHISTOSOMES
What is the scientific name of the body of a tapeworm
STROBILA
The gravid segments of a tapeworm are filled with:
EGGS
A six-hooked embryo is known as:
HEXACANTH
“Ground itch” occurs in infections of hookworms ate the:
SITE OF LARVAL PENETRATION OF THE SKIN
Direct examination of stool specimens stained with iodine makes the trophozoite of the Protozoa stain what color?
TROPHOZOITES ARE DESTROYED BY IODINE
What color does the cytoplasm of protozoan cysts stain in an iodine wet stool preparation:
YELLOW-BROWN
What color do chromatin bodies of protozoan cysts stain in an iodine wet stool preparation:
DO NOT STAIN
What is the advantage of the sedimentation method of concentrating stool specimens?
ALL EGGS WILL SEDIMENT
RECOVERS OPERCULATED EGGS
FECAL DEVRIS AND SOLUBLE MATERIAL CAN BE REMOVED
Trophozoite structures of protozoans, when stained with trichrome stain, appear:
GLYCOGEN- COLORLESS
NUCLEUS- RED PURPLE
CHARCOT-LEYDEN CRYSTALS- BRIGHT RED
Whem stained with Trichrome stain, chromatiod bodies appear what color?
BRIGHT TO RED
The preservative to be employed with the trichrome stain is:
POLYVINYL ALCOHOL
The ideal temperature at which to hold a fecal specimen for more that 1 hour is:
REFRIGERATOR TEMPERATURE
The preferred specific gravity of zinc sulfate solution for the floatation method:
1.18
What type of blood specimen is preferred for preparing blood smears for protozoa
FINGER PUNCTURE
What will happen if the blood from a finger stick mixes with the alcohol used to clean the area?
“FIXES” RBCS
Thick blood films become unsuitable for staining
MALARIA, BABESIA, HEMOFLAGELLATES
To detect stippling, prepare blood films venipuncture within
30 MINUTES TO 1 HOUR AFTER BEING DRAWN

MYCOLOGY QUESTIONS
Charasterictically, fungi
ARE MEMBERS OF THE PLANT KINGDOM
LACK ROOTS AND STEMS
LACH CHLOROPHYLL
Coenocytic hyphae is a synonym forL
ASEPTATE HYPHAE
Which type of mycoses produce no cellular response by the host
SUPERFICIAL MYCOSES
Arthroconidia on the outside of the hair as well as hyphae within the hair shaft is called:
ECTOTHRIX
True yeast produce which of the following in the asexual stage
BLASTOCONIDIA
Septate hyphae have
DIVISIONS
Molds with aseptate hyphae produce a specialized hyphal structure called a
SPORANGIOPHORE
The basis, branching, intertwining structures of molds are referred to as:
MYCELIUM
Arthroconidia are formed:
DIRECTLY FROM THE HYPHAE BY FRAGMENTATION
Mycelia within the colony that grow into the substrate are called:
VEGETATAIVE HYPHAE
Chlamydospores that grow within the hyphae are referred to as:
INTERCALARY
Budding forms in which the daughter cell forms as a bud from a single mother cell or forms laterally from a mycelium of pseudomycelium are called:
BLASTOSPHORE
Which of the following are the result of asexual reproduction:
ASCOSPORE
Conidia are:
ASEXUAL SPORES
Macroconidia are usually:
MULTICELLULAR
The swollen portion of the conidiophores is called the:
VESICLE
Which of the following statements does not apply to true yeasts:
THE EXHIBIT THERMAL DIMORPHISM
Conidia with rough or spiny surfaces are described as:
ECHINULATE
Conidia in chains are said to be:
CATERNATE
When preparing temporary mounts of fungal elements, why is the slide gently heated?
INCREASES THE RATE OF CLEARING
Which of the following acts as a clearing agent to eliminate devris and make fungal elements more prominent in skin, hair and nails?
10% KOH
What is the stain in Aman medium?
COTTON BLUE
Which of the following is negative stain?
INDIA INK STAIN
In the Periodic Acid Schiff (PAS), what colors denote the presence of glycogen?
RED TO VIOLET
Which of the following stains us ised for sharp delineation of fungal elements by fluorescent microscopy?
CALCOFLUOR WHITE
When the fungus wall is invisible with the hematoxylin and eosin stain, which of the following may  be used to restain the slide?
PERIODIC ACID-SCHIFF STAIN
Fungal elements in the acridine orange staining procedure:
FLUORESCES A GREEN COLOR
How does MYCOSESL medium differ from Sabouraud dextrose agar?
CONTAINS CHLORAMPHENICOL AND CYCLOHEXIMIDE
Which fungus medium is used for the primary isolation and maintenance of fungal cultures?
SABOURAUD DEXTROSE AGAR
What is the indicator in Dermatophyte Test Medium?
PHENOL RED
If 1% glucose is added to cornmeal agar, Trichophyton rubrum can be differentiated from Trichophyton mentagrophytes by the production of?
PIGMENT
Fungi should be incubated at
25-30C
How long should fungus cultures be held before reporting as negative?
30 DAYS
Which of the following antibiotics can be added to media for the isolation of pathogenic fungi from contaminated materials?
CYCLOHEXIMIDE AND CHLORAMPHENICOL
Which of the following media stimulates the production of chlamydospores
CORNMEAL AGAR
Which of the following media identifies species of Aspergillus?
CZAPEK’S AGAR
A positive result for the hair-baiting test is:
V-SHAPED PENETRATION OF THE HAIR SHAFT
A tinea infection is commonly referred to as:
RINGWORM
Which of these organisms is the usual cause of white piedra?
TRICHOSPORON BEIGELI
Tinea unguium is a dermatophyte infection of the:
NAIL PLATES
The favus type of tinea capitis is caused by which of the following?
TRICHOPHYTON SCHOENLEINII
Which of the following is/are anthrophilic?
MICROSPORUM AUDOUINII
Hairs infected with which of these dermatophytes fluoresce under Wood’s lamp?
MICROSPORUM CANIS
Colonization by dermatophytic fungi of the hari, nails and skin is called:
DERMATOPHYTOSES
Which of the following fungi os/are the cause of dermatophytoses?
EPIDERMOPHYTON; TRICHOPHYTON, MICROSPORUM
Which of the following produces macroconidia that are large, multicellular and club-shaped with smooth walls?
EPIDERMOPHYTON FLOCCOSUM
Which statement is NOT true?
TRICHOPHYTON RUBRUM IS NOTED FOR ITS “BALLOON” FORMS
Which of the following produces small, very slow growing colonies and has favic chandeliers?
TRICHOPHYTON SCHOENLEINII
Which of the following is diagnostic for chromoblastomycosis?
SCLEROTIC BODY
Which of the following are found in the yeast phase of Sporothrix schenckii on culture medium at 37 C
CIGAR-SHAPED YEAST CELLS
Which of the following is the result of antigen-antibody reaction in cases of sporotrichosis?
ASTEROID BODY
In older mold cultures of Sporothrix schenkii, single conidia borne aling the sides of the hyphae are referred as:
SLOEVE
Which of the following infections is caused by the dematiaceous saprobes that invade organs of immunosuppressed hosts:
PHAEOHYPHOMYCOSIS
The most common cause of mycetoma (maduramycosis) in the U.S is:
PSEUDOALLESCHERIA BOYDII
Dematiaceous fungi have colonies with:
SURFACE AND REVERSE SIDE DARK
Which of the following are a dematiaceous fungi:
PHIALOPHORA AND CADOSPORIUM
Sclerotic bodies are found in tissue in an infection with:
CLADOSPORIUM
Infection of chromoblastomycosis is NOT caused by which of the following fungi?
EXOPHIALA
A fungus that grows as a mold at 35-37 C and a mod at 25 C is described as
MONOMORPHIC
True fungi that produce hyphae and spore forms are known as
EUMYCOTIC
Which of the following causes an infection by hyaline saprobes that invade most tissues or body fluids of immunosuppressed hosts?
ASPARGILLUS; PENICILLIUM MARNEFFEL; FUSARIUM MONLLIFORME
If you observe cleistothecia containing ascospores, report the organism as
PSEUDOALLESCHERIA BOYDII
The actinomycetes are vest classified as:
FUNGUSLIKE BACTERIA
Which of the following is anaerobic, gram-positive but not acid-fast, and does not stain with fungal stain?
ACTINOMYCES ISRAELII
Which of the following has a capsule?
CRYPTOCOCCUS NEOFORMANS
When culturing Cryptococcus on Sabouraud dextrose agar, which of the following antibiotics should not be in the medium?
CYCLOHEXIMIDE
In the direct microscopic examination of cerebrospinal fluid, which of the following can be mistaken for a lymphocyte?
CRYPTOCOCCUS
Why does Cryptococcus neoformans produce a brown color on birdseed or staib agar?
ASSIMILATES CREATININE
Cryptococcus neoformans has the following biochemical reaction/s:
UREASE POSITIVE; INOSITOL POSITIVE; NITRATE POSITIVE
Cryptococcus neoformans latex agglutination test on spinal fluid detects cryptococcal:
ANTIGEN
Candida albicans produces:
BLASTOSPORES; CHLAMYDOSPORES; PSEUDOHYPHAE
Which of the following is another name for oral candidiasis?
THRUSH
A presumptive identification of Candida albicans can be made by checking the ability of the organsm to produce what structure with serum?
GERM TUBE
Cornmeal agar plus Tween 80 is used to identify Candida albicans through the organism’s production of:
CHLAMYDOSPORES
Geotrichum candidum colonies appear as:
RAPID, YEASTLIKE GROWTH
Laboratory workers are in no danger when handling which form of dimorphic fungi?
YEAST FORM (TISSUE)
Sepedonium produces spiny, tuberculate macroconidia but is a:
MONOMORPHIC MOLD
Which of the following are produced by Geotrichum candidum?
RECTANGULAR, EVENLY STAINING ARTHROCONIDIA
Which of the following is/are dimorphic fungi?
HISTOPLASMA CAPSULATUM
BLASTOMYCES DERMATITIDIS
PARACOCCIDIOIDES BRASILIENSIS
The tissue phase if Histoplasma capsulatum, characteristically shows:
YEAST CELLS WITHIN MACROPHAGES
The characteristic structures of the mycelia phase of Histoplasma capsulatum are:
TUBERCULATE CHLAMYDOSPORES
Leishmania can be differentiated from Histoplasma because Leishmania has:
CENTRAL NUCLEAR MATERIAL
Which of the following tests may be sued instead of conversion when identifying dimorphic fungi?
EXOANTIGEN TEST
In the mycelia form, Blastomyces dermatitidis produces:
WIDE, SEPTATE HYPHAE
A yeast form with as ingle broad-based bud would identify an organism as
BLASTOMYCES
The characteristic structures of the mycelia phase of blastomycosis are:
SMALL, PEAR-SHAPED CONIDIA CALLED LOLLIPOPS
Yeastlike forms with multiple buds are characteristic form associated with:
PARACOCCIDIOIDES BRASILIENSIS
Immature spherules of Coccidiodes are differentiated from yeast forms of Blastomyces because:
THEY NEVER BUD
Which of the following fungi is a major biohazard to laboratory personnel?
COCCIDIOIDES IMMITIS
The exoantigen test is a/an
MICROSCOPIC IMMUNODIFFUSION TEST
In the exoantigen test for Histoplasma capsulatum, which bands are present in a positive result?
H AND/OR M
Which of the bands in the exoantigen test for Blastomyces dermatitidis is present in a positive result?
A
Which of the following fungi produces a spherule that is filled with endospores?
COCCIDIOIDES IMMITIS
Zygomycosis is caused by which of the following genera?
MUCOR AND RHIZOPUS
Fungal elements in zygomycosis show hyphae that are:
BROAD AND NONSEPTATE

Bacteriology Questions
Capsules can be used for a :
SEROTYPING BY SWELLING
To demonstrate capsules, grow the culture on media containing:
ANTIBIOTICS AND MILK
The Quellung test depends on the antigenic specificity of the:
CAPSULE
Dark staining granules are called:
METACHROMATIC
Organisms that vary in size and shape are referred to as:
PLEOMORPHIC
In the bacterial growth cycle, growth ceases because nutrients are exhausted or toxic metabolic products have accumulated in the:
STATIONARY PHASE
Which genera of bacteria form spores:
BACILLUS AND CLOSTRIDIUM
Bacteria that grow in the absence of atmospheric (free) oxygen and obtain oxygen from oxygen-containing compounds are called:
ANAEROBES
The temperature at which bacteria grow best is known as:
OPTIMUM
A slimy colony on blood agar medium indicates which of the following characteristics may be present:
ORGANISM HAS CAPSULE
On blood agar plates a small zone of alpha hemolysis surrounded by zone of beta-hemolysis after refrigeration is known as:
ALPHA-PRIME
When using fractional sterilization the sequence is:
HEATING (KILLS VEGETATIVE BACTERIA)
INCUBATION (SPORES GERMINATE)
HEATING (KILLS THE REMAINING VEGETATIVE BACTERIA)
What is the most effective method of sterilization?
AUTOCLAVE
Which of the following is used for quality control for the dry heat oven?
BACILLUS SUBTILIS
Which of the following filters can give 100% sterility?
MILLIPORE (0.22 UM)
Media that cannot be heated can be sterilized by:
FILTRATION
Which of the following is not a disinfectant?
70% ALCOHOL
Iodophores are composed of iodine and?
DETERGENT
Quaternary ammonium disinfectants are easily inactivated by:
ORGANIC MATERIAL
What is a term that describes a process or treatment that renders a medical device, instrument, or environmental surface safe to handle?
DECONTAMINATION
What do gram-positive bacteria stain?
PURPLE
The mordant used in Ziehl-Neelsen acid fast is:
HEAT
How is smear for an acid fast stain fixed?
SLIDE WARMER AT 65 C FOR 2 HOURS
Acid fast bacteria appear what color microscopically?
RED
Acid fast organisms are best stained by which of the following methods?
KINYOUN
In the Ziehl Neelsen staining method, the decolorizer is:
ALCOHOL+HCL
An example of a negative stain is:
INDIA INK STAIN
Which of the following is not a gram negative?
CAMPYLOBACTER
If a patient is taking antimocrobials, which of the following can neutralize the antimicrobials?
THIOL BROTH
From a bronchial washing, organisms are seen on a Gram stain, but no growth occurs aerobically and anaerobically. This is most likely due to:
INHIBITION BY ANTIBIOTIC THERAPY
Which of the following should be used to remove antimicrobials before culturing?
ANTIMICROBIAL REMOVAL DEVICE
Sodium polyanetholsulfonate may be used as an anticoagulant in blood culture because it:
PREVENTS PHAGOCYTOSIS
NEUTRALIZES THE BACTERICIDAL EFFECT OF HUMAN SERUM
What is the most abundant normal flora in throat cultures?
ALPHA-HEMOLYTIC STREPTOCOCCUS
Why must blood plates for throat cultures be incubated aerobically and anaerobically when beta hemolytic strepotococci are suspected?
SOME MAY PRODUCE BETA HEMOLYSIS UNDER AEROBIC CONDITIONS
Nasopharyngeal swabs are recommended for the detection of carriers such as:
HAEMOPHILUS INFLUENZA
NEISSERIA
BORDETELLA PERTUSSIS
Todd-Hewitt broth is recommended for:
CULTURE OF BETA HEMOLYTIC STREPTOCOCCI FOR FLUORESCENCE MICROSCOPY
Phenylethyl alcohol is used in media to:
INHIBIT GRAM NEGATIVE BACTERIA
Why is a first morning urine specimen preferred for urine culture?
SPECIMEN IS MORE CONCENTRATED
What indicates vaginal or urethral contamination of urine?
MANY SQUAMOUS EPITHELIAL CELLS

On the pour plate method for colony counts, how does the amount of agar added affect the dilution?
DILUTION IS NOT AFFECTED
Smears of cerebrospinal fluid are prepared from:
CEREBROSPINAL FLUID SEDIMENT
Smears for CSF are usually stained with:
GRAM STAIN AND INDIA INK STAIN
Which of the following tests for bacterial antigen on cultures isolated from CSF is more sensitive and faster?
LATEX AGGLUTINATION TESTS
Staphylococcal protein A coated with antiserum is used in which of the following serological tests on CSF?
COAGGLUTINATION TEST






Which of the following allows viability of acid production oxidizers in oxidation-fermentation medium?

Which of the following is oxidase positive?

Which of the following is the reagent used for the string test?

Any organism that is indole positive and nitrate reduction positive is also:

How many tubes of oxidation fermentation media is/are inoculated and what precautions should be taken?

Whicj of the following is oxidase positive?

Which of the following is part of the normal flora of the skin?

What does PRAS mean?

Why should thioglycollate both be boiled for 10 minutes before being used?

Nagler agar is a selective medium for?

Peptococcus and Pepetostreptococcus are both:

Propionibacteria are:

A gram negative anaerobic coccus that produces a red fluorescence under ultraviolet light is:

Which anaerobic, gram positive rods produce terminal “lollipop” spores?

Which of the following anaerobes causes an antimicrobial associated diarrhea?

Which of the following clostridia produce a double zone of hemolysis around colonies on blood agar?

Mycoplasma pneumonia is incubated

A positive urease test for ureaplasma is indicated by a:

Which of the following organinsms produces no haze in a broth culture?

What is the cause of primary atypical pneumonia?

Which of the following has both ribonucleic acid and deoxyribonucleic acid?

Rickettsias are transmitted by:

Which of the following is not caused by Rickettsia?

Which of the following have a bacterial type cell wall?

Which of the following is a small nonmotile, coccobacillus that is cultured in yolk sac of a chick embryo?

Chlamydia are cultured in:

Genital cultures for sexually transmitted diseases include all of the following except:

TRIC conjunctivitiss include all of the following:

Which serological test is done for Chlamydia trachomatis on genital smears?

What is the common tick-borne disease in the U.S?

What is the erythema chronicum migrans (ECM)?

What are broad spectrum antibiotics?

Substances produced by microorganimsms, that in very small amounts inhibit other microorganisms are called?

Bacteriostatic agents do which of the following?

The time it takes half a dose of antibiotics to disappear from the bllod is called:

Resistance to an antibiotic can be transferred from a resistant ti a susceptible organism by:

In the Kirby-Bauer susceptibility test, the 0.5 McFarland standard is used to:

After inoculating the Mueller-Hinton plates for the Kirby-Bauer susceptibility test, how long should the plates dry before adding the disks?

How does one measure the zone of growth for inhibition for the Kirby-Bauer susceptibility test?

What does the size of the zone of growth inhibition correlate with if the correct procedure has been used?

If there is too much moisture on the surface of a Mueller-Hinton plate when performing a Kirby-Bauer susceptibility test, what will happen to the growth?

Which of the following are the best indicators for poor storage?

In Mueller Hinton agar, what can cause increase resistance of Pseudomonas aeruginosa to aminoglycosides?
INCREASED CALCIUM AND MAGNESIUM
When doing a susceptibility test with sulfonamides, there are two concentric zones around the disk. How should the zone be measured?
MEASURE THE DIAMETER OF THE OUTER ZONE
The lowest concentration of drug that will kill all but the minimum defined proportion of viable organisms after incubation for a fixed time under a given set of conditions is the definition of:
MINIMUM LETHAL CONCENTRATION
MINIMUM BACTERICIDAL CONCENTRATION
For quality control when monitoring reagents, how often should reagent disks be checked?
WHEN CONTAINER IS FIRST OPENED
ONCE EACH WEEK OF USE
How often should catalase, oxidase and coagulase reagents be tested?
ONCE EACH DAY OF USE
WHEN VIAL IS FIRST OPENED
Record temperatures of incubators, water baths, heating blocks, refrigerators, freezers and thermometers
AT EACH TIME OF USE
AT THE BEGINNING OF EACH DAY
AT THE END OF EACH DAY
Check the face velocity of safety cabinets each
MONTH
Setting of rpms marked on the face of rheostat control on the centrifuge should be checked once:
MONTHLY