Medical Technology Board Examination Review Notes 5

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A mature virus particle containing a nucleic acid core surrounded by a protein coat, with or without envelope, is called:

 

VIRION

Viruses are characterized by the presence of:

DNA AND RNA

What is the largest DNA virus?

POXVIRUS

Which is the smallest RNA virus?

ENTEROVIRUS

What is the first step in the replication cycle of a virus?

ATTACHMENT AND PENETRATION

The virus capsid morphology is:

HELICAL OR ICOSAHEDRAL

From what part of the virus is the envelop acquired?

NUCLEAR OR CYTOPLASMIC MEMBRANE

Where is the site of virion assembly?

NUCLEUS OR CYTOPLASM

Which of the following is a DNA virus?

HERPESVIRIDAE

Which of the following is an RNA virus?

PSEUDOMYXOVIRIDAE

Specimens collected for virus isolation should be kept at:

4C

At what temperature should clinical specimens suspected of containing viruses kept for transport that takes days?

-70C

In what family of viruses, after primary infection, does an individual become latently infected and then the infection can be reactivated?

HERPESVIRIDAE

Cytomegalovirus isolation is best accomplished using:

HUMAN EMBRYONIC FIBROBLASTS

Which of the following viruses causes acute central nervous system disease in humans and animals?

RABIES

What specimen/s should be collected from a patient with suspected enteroviral meningitis?

STOOL, THROAT SWAB, CSF

Influenza A viruses can be detected in cell cultures by:

HEMADSORPTION ASSAY

The best host systems for influenza virus isolation are:

MONKEY KIDNEY CELLS AND EMBRYONATED HEN’S EGGS

What common antigen is cross-reactive in all human adenoviruses?

HEXON

The following characteristics are similar in both influenza and parainfluenza viruses

HELICAL SYMMETRY, LIPID ENVELOPE, BUDDNG AND CYTOPLASMIC MEMBRANE

Myxoviridae virus envelope contains both:

NEURAMINIDASE AND HEMAGGLUTININ

Eosinophilic cytoplasmic inclusions are elemtary bodies of the:

POXVIRUS

German measles is caused by:

RUBELLA VIRUS

What is an alastrim infection?

MILD FORM OF SMALLPOX

Which of the following procedure is routinely used for the detection of Hepatitis B virus (HBV) in blood donors?

SOLID-PHASE RIA AND ELISA

Which of the following laboratory procedures is the most rapid method for diagnosis of a virus infection?

DIRECT ELECTRON MICROSCOPY

What specimens should be collected from a patient with suspected influenza?

THROAT SWAB

What virus group contains a double stranded, segmented RNA genome?

REOVIRUS

Enterovirus can be differentiated from rhinoviruses by:

ACID RESISTANCE

What virus is the most frequent cause of gastroenteritis in children during the winter months?

ROTAVIRUS

All of the following groups of viruses are resistant to ether treatment except:

HERPESVIRUSES

The most common method for rapid electron microscopy examination is:

NEGATIVE STAINING

Various virus groups can be differentiated in negative stained specimens by their:

MORPHOLOGY

PARASITOLOGY QUESTIONS

The motile, reproducing stage, feeding stage of the Protozoa is:

TROPHOZOITE

Which of the following amoeba has chromatoid bodies in the cyst stage?

ENTAMOEBA

Amebiasis is caused by

E. HISTOLYTICA

Which of the following organs of the body is most often involved in extraintestinal amebiasis?

LIVER

Which of the following is characteristic of true amebiasis?

- PERIPHERAL CHROMATIN ON THE NUCLEAR BODY

- CHROMATIN BODIES IN THE CYST

- INCLUDE PATHOGENS AND NONPATHOGENS

Which of the following is a true ameba?

E. HARTMANNI

Amebae that inhabits the gastrointestinal tract of man are nonmotile, nonfeeding, and infective during which stage?

CYST

Mature cysts of E. polecki have how many nuclei?

1

Which of the following has cysts with chromatoid bodies that have two pointed ends or that can be round, triangular or oval?

E. COLI

Trophozoites of Entameoba histolytica have the following characteristics:

- SMALLL, DELICATE NUCLEAR CHROMATIN

- FINE, EVEN, PERIPHERAL CHROMATIN

- PROGRESSIVE MOTILITY WITH HYALINE, FINGER-LIKE PSEUDOPODS

Which of these trophozoites, when acting as a pathogen, is likely to ingest red blood cells of the host?

E. HISTOLYTICA

The point of differentiation between Entamoeba histolytica and Entamoeba hartmanni is?

SIZE

The mature cyst of Entamoeba histolytica has how many nuclei?

FOUR

Failure to find bacteria in purulent spinal fluid should alert one to find the possibility of an infection with?

AMEBAE

Which trophozoite is distinguished by the possession of achromatic granules surrounding its karyosomal chromatin?

I. BUTSCHLII

Which of the following would have double-walled, wrinkled cyst form?

A. CASTELLANII

A cyst that possesses a single nucleus and a large glycogen vacuole that stains deeply with iodine belong to:

I. BUTSCHLII

Some amebae have spiny, hyaline extensions called:

ACANTHOPODS

Which are the best staining procedures for species of Naegleria and Acanthamoeba?

H&E  AND WRIGHT’S STAIN

Amebae inhabiting the central nervous system enter the body through?

NASAL MUCOSA

In which specimen are Acanthamoeba and Naegleria usually found?

CEREBROSPINAL FLUID

Naegleria has which of the following characteristics

- FOUND IN THE BRAIN

- TROPHOZOITE CAN ASSUME A LIMAX FORM

- TROPHOZOITE CAN BECOME AN AMEBA FLAGELLATE

The trophozoite whose karyosomal chromatin appears as a rosette of 4-6 granules is identified as:

N. FOWLERI

Which of the following are often mistaken for cysts of amebae?

BLASTOCYSTIS HOMINIS

Intestinal flagellates are usually which shape in the trophozoite stage?

PEAR-SHAPED

Which of the following is pathognomonic for G. lamblia and the stage it is found in?

VENTRAL SUCKING DISK- TROPHOZOITE

Which of the following protozoa have an undulating membrane?

TRICHOMONAS, TRYPANOSOMA

Infections with D. fragilis can show which of the following symptoms?

DIARRHEA

ABDOMINAL DISCOMFORT WITHOUT DIARRHEA

ASYMPTOMATIC

What does D. fragilis have in common with T. vaginalis?

NEITHER HAS CYST FOR

Which flagellate can be a pathogen of small intestines?

GIARDIA

Which intestinal flagellate trophozoite has a sucking disk, 2 nucleo, 8 flagella and an axostyle?

G. LAMBLIA

Red and white blood cells in stool specimens are characteristic of?

BACILLARY DYSENTERY

A pear-shaped flagellate with jerky motility that is found in a urine specimen is identified as?

T. VAGINALIS

Eighty percent of the trophozoites of D. fragilis have?

2 NUCLEI

The only bilaterally symmetrical protozoan is?

GIARDIA

Whoch of the following is the intracellular form of blood and tissue flagellates?

LEISHMANIAL

Which of the following is the cause of African sleeping sickness?

TRYPANOSOMA

Which of the following is the vector of African sleeping sickness?

TSETSE FLY

Which species of Trypanosoma is the cause of Chagas disease?

CRUZI

Which of the following is characteristic of the trypanosome form of Trypanosoma cruzi?

PROMINENT KINETOPLAST

C-SHAPED

THICK ORGANISM

Which specimen would be suitable for a demonstration of the trypanosomes of sleeping sickness?

BLOOD

FLUID FROM LYMPH NODE

CSF

The extracellular form of Trypanosoma is a slender organism characterized by an undulating membrane and a free flagellum that arises:

POSTERIORLY FROM THE KINETOPLAST

Which of the following is found within the reticuloendothelial cells?

L. DONOVANI

H. CAPSULATUM

T. GONDII

How is the Trypanosoma cruzi transmitted?

BITE OF REDUVIID BUG

A chagoma is a lesion deen in infections with:

TRYPANOSOME CRUZI

Which organism is the cause of kala-azar?

L. DONOVANI

In the laboratory diagnosis of L. donovani, which is the preferred specimen in which to find Leihman-Donovan bodies?

BONE MARROW

Which of the following is the only ciliate that is pathogenic in humans?

B COLI

A very large cyst whose double wall encloses a ciliated organism with one visible nuclei would be:

ACQUIRED BY ACCIDENT BY MAN, SINCE IT CUSTOMARILY INFECTS SWINE

Which of the following structures are used for the motility of Balantidium coli?

CILIA

Which nucleus in the trophozoite of Balantidium coli is the reproductive one?

MICRONUCLEUS

Conjugation of trophozoites of Balantidium coli never occurs between:

SAME SIZE ORGANISMS

The definitive host (vector) to Plasmodium is the:

FEMALE ANOPHELES MOSQUITO

Which of the malarial organisms presents as pale, very ameboid ring trophozoites, infecting a large pale red blood cell with dots of hemoglobin?

P. VIVAX

Which of the malarial organisms preferentially invades reticulocytes?

P. VIVAX

The malarial organism whose schizont resembles a “fruit pie” in which the merozoite form a rosette around the malarial pigment is:

MALARIAE

The malarial organism characteristically has a band form trophozoite stretching across the red blood cell?

P. MALARIAE

The gametocyte of Plasmodium falciparum can be differentiated from that of other malarial species by?

SHAPE

In which type of malaria can Ziemann’s stippling be found?

MALARIAE

In which type of malaria can Maurer’s dots be found?

P. FALCIPARUM

Which malarial organism has large, coarse, red dots with a large, pale red blood cell with fimbriated edges?

P. OVALE

The sexual reproduction cycle in Plasmodiu, and Coccidia is referred to as:

SPOROGONY

What is the infective stage of the malarial parasite to the vector?

GAMETOCYTE

What is the infective stage of the malarial parasite to humans?

SPOROZOITES

In which type of malaria is there synchronized rupture of the red blood cells every 72 hours?

MALARIAE

In falciparum malaria, there may be a sudden massive intravascular hemolysis producing hemoglobinuria. This is called:

BLACKWATER FEVER

Which hemoglobin is incompatible with malarial parasite survival?

HB-SS

What is the name of the laboratory test that allows laboratory bred reduviid bugs to feed on patients suspected of having Chagas’ disease?

XENODIAGNOSIS

 

Human are infected with Babesia by:

BITE OF TICKS

BLOOD TRANSFUSION

If immature oocysts of Isospora belli are found in stool specimens from infected humans, what should be done with the specimen for identification?

LEAVE AT ROOM TEMPERATURE

What stage of Isospora is infective to humans?

OOCYSTS

Isospora belli immature oocysts contain/s:

SPOROBLAST

Infective oocysts of Isospora belli contain:

SPOROZOITES

The demonstration of retinochoroiditis and cerebral calcifications in a newborn would result in which of these laboratory requests:

TITER OF TOXOPLASMA ANTIBODIES

What is both the definitive and intermediate host of Toxoplasma gondii:

CAT

What is the appearance of Toxoplasma gondii in tissue fluids of man?

CRESCENT

What constitutes a positive result in the Sabin-Feldman dye test?

TOXOPLASMA LOSES ITS AFFINITY FOR METHYLENE BLUE DYE

Which of the following tests is used for the detection of Cryptosporidium?

SHEATHER’S SUGAR FLOATATION

Which of the following parasites poses a particular hazard for immunodeficient or immunosuppressed individuals?

CRYPTOSPORIDIUM, GIARDIA, STRONGYLOIDES

Which of the followi0ng parasites is associated with AIDS?

CRYPTOSPORIDIUM

Which operculated ovum contains a miracidium and can appear in sputum, often accompanied by blood and Charcot-Leyden crystals?

PARAGONIMUS WESTERMANI

Which of the schistosomes may be recovered in rectal biopsy?

SCHISTOSOMA MANSONI AND SCHISTOSOMA JAPONICUM

The scientific name of the head of a tapeworm is:

SCOLEX

Which of the following worms have an oral and ventral shaped sucker in the adult stage?

TREMATODES

Which of the following are hermaphroditic?

FLUKES AND ROUNDWORMS

Which of the following is the first intermediate host of the flukes?

SNAIL

The common names for the schostosomes is:

BLOOD FLUKES

What is the common name for Clonorchis sinensis?

CHINESE LIVER FLUKE

What is a schistosomule?

CERCARIAE MINUS TAIL

The beef tapeworm is the common name for?

T. SAGINATA

Which tapeworm proglottid makes its way across the fecal specimen by doubling movements, and is seen under the microscope to have numerous regular uterine branches (more that 15) resembling those of a tree?

T. SAGINATA

Which of the following has an unarmed scolex?

BEEF TAPEWORM

Which species of Taenia has 7 to 12 uterine branches?

T. SOLIUM

A hexacanth embryo enclosed in a radially striated shell belongs to the genus

TAENIA

Which tapeworm infection has to be treated with great care so that man does not acquire the larval infection:

TAENIASIS SOLIUM

A bile stained egg that is 75 microns at its greatest diameter and contains a hexacanth embryo that lacks polar knobs or filaments is that of:

H. DIMINUTA

The “dwarf” tapeworm is another name for:

H. NANA

The eggs of Echinococcus granulosus are found in the feces of:

DOGS

Which of the following is found in the intermediate host of Echonococcus granulosus?

HYDATID CYST

Hydatid cyst infection of man is due to a larva of the tapeworm:

E. GRANULOSUS

The first intermediate host of Diphylloborhrium latum is:

COPEPOD

The second intermediate host of Diphylloborhrium latum is:

FRESHWATER FISH

What is the infective stage of the broad fish tapeworm to humans?

PLEROCERCOID

The eggs of this tapeworm are developed and have an operculum at one end and a small abopercular knob at the other end. The tapeworm is:

D. LATUM

In some individuals, particularly those of Scandinavian extraction, this worm can cause megaloblastic anemia.

D. LATUM

Which of the following worms have separate sexes

BLOOD FLUKES

The male roundworm is differentiated from the female roundworm by its:

DORSALLY CURVED POSTERIOR

The best way to demonstrate a pinworm infection is by which of the following techniques?

CELLOPHANE TAPE PREPARATION

The gravid female of Enterobius vermicularis deposits her embryonated eggs:

ON THE PERIANAL SKIN

Eggs that average 60 microns in lenghth, are oval with one flattened side, and contain a motile larva, are those of:

E. VERMICULARIS

Eggs that are bile-stained and have clear polar plugs belong to the:

WHIPWORM

Which of the following nematodes does NOT have a free-living state:

E. VERMICULARIS

Whicj stage of Trichuris trichiura is infective to humans?

EMBRYONATED EGG

Where does the larva of Ascaris lumbricoides go after it hatches In the small intestines?

MIGRATES THROUGH THE BLOOD, LIVER, LUNGS, PHARYNX, AND THEN BACK TO THE SMALL INTESTINES

If an Ascaris egg lacks its bile-stained mamillated coat, we refer to the egg as:

DECORTICATED

Trichuris trichiura, hookworm and ____ form the “unholy three” of roundworms:

A. LUMBRICOIDES

Which of the following organisms caused infections where the larvae migrate into the lymphatics and blood, lung, alveoli, bronchiolus, pharynx, and then the small intestines:

N. AMERICANUS,

 A. DUODENALE

S. STERCOLARIS

Which of the following is characteristic of the eggs of hookworms?

AN EMBRYO I0N THE TWO-TO-EIGHT CELL STAGE OF CLEAVAGE

OVA, THIN SHELL

CLEAR SPACE BETWEEN SHELL AND EMBRYO

The helminth that induces a hypochromic microcytic anemia in its host is the:

HOOKWORM

The rhabtidiform larva of the hookworm has a:

LONG BUCCAL CAVITY EQUAL TO THE WIDTH OF THE BODY

Which of the following larval worms enters the host by penetration of the skin?

S. STERCORALIS; A. DUODENALE; N. AMERICANUS

A roundworm that ingabits the small intestine and usually is demonstrated as rhabditiform larvae in the fecal specimen is the:

THREADWORM

Muscle biopsy is a diagnostic technique employed to detect:

TRICHINOSIS

Which of the following roundworms give birth to her young:

WUCHERERIA; MANSONELLA; BRUGIA

A viviparous female nematode:

PRODICE LIVING LARVAE

One of the differential characteristics of the microfilariae is the presence or absence of:

A SHEATH

Adults of filarial worms live in:

LYMPHATICS AND SUBCUTANEOUS TISSUE

One of the pathological findings in infestations of Wuchereria is:

ELEPHANTIASIS

The intermediate host of the guinea worm is the:

COPEPOD

Dirofilaria immitis is the:

DOG HEARTWORM

Dracunculus infections cause ulcers that:

ON CONTACT WITH WATER, RELEASE LARVAE

Which of the following microfilariae does NOT have a sheath

MANSONELLA

Which of the following is the eye worm

LOA

Cutaneous larva migrans or creeping eruption is caused by:

FILARIFORM LARVAE OF DOG HOOKWORM

Which of the following causes a chronic cough, pulmonary and artery obstruction but no invasion of the heart in humans?

DIROFILARIA IMMITIS

Which of the following is caused by the ingestion of snails by humans?

RAT LUNGWORM

Cercarial dermatitis, commonly known as swimmer’s itch, is caused by:

CERCARIAE OF SCHISTOSOMES

What is the scientific name of the body of a tapeworm

STROBILA

The gravid segments of a tapeworm are filled with:

EGGS

A six-hooked embryo is known as:

HEXACANTH

“Ground itch” occurs in infections of hookworms ate the:

SITE OF LARVAL PENETRATION OF THE SKIN

Direct examination of stool specimens stained with iodine makes the trophozoite of the Protozoa stain what color?

TROPHOZOITES ARE DESTROYED BY IODINE

What color does the cytoplasm of protozoan cysts stain in an iodine wet stool preparation:

YELLOW-BROWN

What color do chromatin bodies of protozoan cysts stain in an iodine wet stool preparation:

DO NOT STAIN

What is the advantage of the sedimentation method of concentrating stool specimens?

ALL EGGS WILL SEDIMENT

RECOVERS OPERCULATED EGGS

FECAL DEVRIS AND SOLUBLE MATERIAL CAN BE REMOVED

Trophozoite structures of protozoans, when stained with trichrome stain, appear:

GLYCOGEN- COLORLESS

NUCLEUS- RED PURPLE

CHARCOT-LEYDEN CRYSTALS- BRIGHT RED

Whem stained with Trichrome stain, chromatiod bodies appear what color?

BRIGHT TO RED

The preservative to be employed with the trichrome stain is:

POLYVINYL ALCOHOL

The ideal temperature at which to hold a fecal specimen for more that 1 hour is:

REFRIGERATOR TEMPERATURE

The preferred specific gravity of zinc sulfate solution for the floatation method:

1.18

What type of blood specimen is preferred for preparing blood smears for protozoa

FINGER PUNCTURE

What will happen if the blood from a finger stick mixes with the alcohol used to clean the area?

“FIXES” RBCS

Thick blood films become unsuitable for staining

MALARIA, BABESIA, HEMOFLAGELLATES

To detect stippling, prepare blood films venipuncture within

30 MINUTES TO 1 HOUR AFTER BEING DRAWN

 

MYCOLOGY QUESTIONS

Charasterictically, fungi

ARE MEMBERS OF THE PLANT KINGDOM

LACK ROOTS AND STEMS

LACH CHLOROPHYLL

Coenocytic hyphae is a synonym forL

ASEPTATE HYPHAE

Which type of mycoses produce no cellular response by the host

SUPERFICIAL MYCOSES

Arthroconidia on the outside of the hair as well as hyphae within the hair shaft is called:

ECTOTHRIX

True yeast produce which of the following in the asexual stage

BLASTOCONIDIA

Septate hyphae have

DIVISIONS

Molds with aseptate hyphae produce a specialized hyphal structure called a

SPORANGIOPHORE

The basis, branching, intertwining structures of molds are referred to as:

MYCELIUM

Arthroconidia are formed:

DIRECTLY FROM THE HYPHAE BY FRAGMENTATION

Mycelia within the colony that grow into the substrate are called:

VEGETATAIVE HYPHAE

Chlamydospores that grow within the hyphae are referred to as:

INTERCALARY

Budding forms in which the daughter cell forms as a bud from a single mother cell or forms laterally from a mycelium of pseudomycelium are called:

BLASTOSPHORE

Which of the following are the result of asexual reproduction:

ASCOSPORE

Conidia are:

ASEXUAL SPORES

Macroconidia are usually:

MULTICELLULAR

The swollen portion of the conidiophores is called the:

VESICLE

Which of the following statements does not apply to true yeasts:

THE EXHIBIT THERMAL DIMORPHISM

Conidia with rough or spiny surfaces are described as:

ECHINULATE

Conidia in chains are said to be:

CATERNATE

When preparing temporary mounts of fungal elements, why is the slide gently heated?

INCREASES THE RATE OF CLEARING

Which of the following acts as a clearing agent to eliminate devris and make fungal elements more prominent in skin, hair and nails?

10% KOH

What is the stain in Aman medium?

COTTON BLUE

Which of the following is negative stain?

INDIA INK STAIN

In the Periodic Acid Schiff (PAS), what colors denote the presence of glycogen?

RED TO VIOLET

Which of the following stains us ised for sharp delineation of fungal elements by fluorescent microscopy?

CALCOFLUOR WHITE

When the fungus wall is invisible with the hematoxylin and eosin stain, which of the following may  be used to restain the slide?

PERIODIC ACID-SCHIFF STAIN

Fungal elements in the acridine orange staining procedure:

FLUORESCES A GREEN COLOR

How does MYCOSESL medium differ from Sabouraud dextrose agar?

CONTAINS CHLORAMPHENICOL AND CYCLOHEXIMIDE

Which fungus medium is used for the primary isolation and maintenance of fungal cultures?

SABOURAUD DEXTROSE AGAR

What is the indicator in Dermatophyte Test Medium?

PHENOL RED

If 1% glucose is added to cornmeal agar, Trichophyton rubrum can be differentiated from Trichophyton mentagrophytes by the production of?

PIGMENT

Fungi should be incubated at

25-30C

How long should fungus cultures be held before reporting as negative?

30 DAYS

Which of the following antibiotics can be added to media for the isolation of pathogenic fungi from contaminated materials?

CYCLOHEXIMIDE AND CHLORAMPHENICOL

Which of the following media stimulates the production of chlamydospores

CORNMEAL AGAR

Which of the following media identifies species of Aspergillus?

CZAPEK’S AGAR

A positive result for the hair-baiting test is:

V-SHAPED PENETRATION OF THE HAIR SHAFT

A tinea infection is commonly referred to as:

RINGWORM

Which of these organisms is the usual cause of white piedra?

TRICHOSPORON BEIGELI

Tinea unguium is a dermatophyte infection of the:

NAIL PLATES

The favus type of tinea capitis is caused by which of the following?

TRICHOPHYTON SCHOENLEINII

Which of the following is/are anthrophilic?

MICROSPORUM AUDOUINII

Hairs infected with which of these dermatophytes fluoresce under Wood’s lamp?

MICROSPORUM CANIS

Colonization by dermatophytic fungi of the hari, nails and skin is called:

DERMATOPHYTOSES

Which of the following fungi os/are the cause of dermatophytoses?

EPIDERMOPHYTON; TRICHOPHYTON, MICROSPORUM

Which of the following produces macroconidia that are large, multicellular and club-shaped with smooth walls?

EPIDERMOPHYTON FLOCCOSUM

Which statement is NOT true?

TRICHOPHYTON RUBRUM IS NOTED FOR ITS “BALLOON” FORMS

Which of the following produces small, very slow growing colonies and has favic chandeliers?

TRICHOPHYTON SCHOENLEINII

Which of the following is diagnostic for chromoblastomycosis?

SCLEROTIC BODY

Which of the following are found in the yeast phase of Sporothrix schenckii on culture medium at 37 C

CIGAR-SHAPED YEAST CELLS

Which of the following is the result of antigen-antibody reaction in cases of sporotrichosis?

ASTEROID BODY

In older mold cultures of Sporothrix schenkii, single conidia borne aling the sides of the hyphae are referred as:

SLOEVE

Which of the following infections is caused by the dematiaceous saprobes that invade organs of immunosuppressed hosts:

PHAEOHYPHOMYCOSIS

The most common cause of mycetoma (maduramycosis) in the U.S is:

PSEUDOALLESCHERIA BOYDII

Dematiaceous fungi have colonies with:

SURFACE AND REVERSE SIDE DARK

Which of the following are a dematiaceous fungi:

PHIALOPHORA AND CADOSPORIUM

Sclerotic bodies are found in tissue in an infection with:

CLADOSPORIUM

Infection of chromoblastomycosis is NOT caused by which of the following fungi?

EXOPHIALA

A fungus that grows as a mold at 35-37 C and a mod at 25 C is described as

MONOMORPHIC

True fungi that produce hyphae and spore forms are known as

EUMYCOTIC

Which of the following causes an infection by hyaline saprobes that invade most tissues or body fluids of immunosuppressed hosts?

ASPARGILLUS; PENICILLIUM MARNEFFEL; FUSARIUM MONLLIFORME

If you observe cleistothecia containing ascospores, report the organism as

PSEUDOALLESCHERIA BOYDII

The actinomycetes are vest classified as:

FUNGUSLIKE BACTERIA

Which of the following is anaerobic, gram-positive but not acid-fast, and does not stain with fungal stain?

ACTINOMYCES ISRAELII

Which of the following has a capsule?

CRYPTOCOCCUS NEOFORMANS

When culturing Cryptococcus on Sabouraud dextrose agar, which of the following antibiotics should not be in the medium?

CYCLOHEXIMIDE

In the direct microscopic examination of cerebrospinal fluid, which of the following can be mistaken for a lymphocyte?

CRYPTOCOCCUS

Why does Cryptococcus neoformans produce a brown color on birdseed or staib agar?

ASSIMILATES CREATININE

Cryptococcus neoformans has the following biochemical reaction/s:

UREASE POSITIVE; INOSITOL POSITIVE; NITRATE POSITIVE

Cryptococcus neoformans latex agglutination test on spinal fluid detects cryptococcal:

ANTIGEN

Candida albicans produces:

BLASTOSPORES; CHLAMYDOSPORES; PSEUDOHYPHAE

Which of the following is another name for oral candidiasis?

THRUSH

A presumptive identification of Candida albicans can be made by checking the ability of the organsm to produce what structure with serum?

GERM TUBE

Cornmeal agar plus Tween 80 is used to identify Candida albicans through the organism’s production of:

CHLAMYDOSPORES

Geotrichum candidum colonies appear as:

RAPID, YEASTLIKE GROWTH

Laboratory workers are in no danger when handling which form of dimorphic fungi?

YEAST FORM (TISSUE)

Sepedonium produces spiny, tuberculate macroconidia but is a:

MONOMORPHIC MOLD

Which of the following are produced by Geotrichum candidum?

RECTANGULAR, EVENLY STAINING ARTHROCONIDIA

Which of the following is/are dimorphic fungi?

HISTOPLASMA CAPSULATUM

BLASTOMYCES DERMATITIDIS

PARACOCCIDIOIDES BRASILIENSIS

The tissue phase if Histoplasma capsulatum, characteristically shows:

YEAST CELLS WITHIN MACROPHAGES

The characteristic structures of the mycelia phase of Histoplasma capsulatum are:

TUBERCULATE CHLAMYDOSPORES

Leishmania can be differentiated from Histoplasma because Leishmania has:

CENTRAL NUCLEAR MATERIAL

Which of the following tests may be sued instead of conversion when identifying dimorphic fungi?

EXOANTIGEN TEST

In the mycelia form, Blastomyces dermatitidis produces:

WIDE, SEPTATE HYPHAE

A yeast form with as ingle broad-based bud would identify an organism as

BLASTOMYCES

The characteristic structures of the mycelia phase of blastomycosis are:

SMALL, PEAR-SHAPED CONIDIA CALLED LOLLIPOPS

Yeastlike forms with multiple buds are characteristic form associated with:

PARACOCCIDIOIDES BRASILIENSIS

Immature spherules of Coccidiodes are differentiated from yeast forms of Blastomyces because:

THEY NEVER BUD

Which of the following fungi is a major biohazard to laboratory personnel?

COCCIDIOIDES IMMITIS

The exoantigen test is a/an

MICROSCOPIC IMMUNODIFFUSION TEST

In the exoantigen test for Histoplasma capsulatum, which bands are present in a positive result?

H AND/OR M

Which of the bands in the exoantigen test for Blastomyces dermatitidis is present in a positive result?

A

Which of the following fungi produces a spherule that is filled with endospores?

COCCIDIOIDES IMMITIS

Zygomycosis is caused by which of the following genera?

MUCOR AND RHIZOPUS

Fungal elements in zygomycosis show hyphae that are:

BROAD AND NONSEPTATE


Bacteriology Questions

Capsules can be used for a :

SEROTYPING BY SWELLING

To demonstrate capsules, grow the culture on media containing:

ANTIBIOTICS AND MILK

The Quellung test depends on the antigenic specificity of the:

CAPSULE

Dark staining granules are called:

METACHROMATIC

Organisms that vary in size and shape are referred to as:

PLEOMORPHIC

In the bacterial growth cycle, growth ceases because nutrients are exhausted or toxic metabolic products have accumulated in the:

STATIONARY PHASE

Which genera of bacteria form spores:

BACILLUS AND CLOSTRIDIUM

Bacteria that grow in the absence of atmospheric (free) oxygen and obtain oxygen from oxygen-containing compounds are called:

ANAEROBES

The temperature at which bacteria grow best is known as:

OPTIMUM

A slimy colony on blood agar medium indicates which of the following characteristics may be present:

ORGANISM HAS CAPSULE

On blood agar plates a small zone of alpha hemolysis surrounded by zone of beta-hemolysis after refrigeration is known as:

ALPHA-PRIME

When using fractional sterilization the sequence is:

HEATING (KILLS VEGETATIVE BACTERIA)

INCUBATION (SPORES GERMINATE)

HEATING (KILLS THE REMAINING VEGETATIVE BACTERIA)

What is the most effective method of sterilization?

AUTOCLAVE

Which of the following is used for quality control for the dry heat oven?

BACILLUS SUBTILIS

Which of the following filters can give 100% sterility?

MILLIPORE (0.22 UM)

Media that cannot be heated can be sterilized by:

FILTRATION

Which of the following is not a disinfectant?

70% ALCOHOL

Iodophores are composed of iodine and?

DETERGENT

Quaternary ammonium disinfectants are easily inactivated by:

ORGANIC MATERIAL

What is a term that describes a process or treatment that renders a medical device, instrument, or environmental surface safe to handle?

DECONTAMINATION

What do gram-positive bacteria stain?

PURPLE

The mordant used in Ziehl-Neelsen acid fast is:

HEAT

How is smear for an acid fast stain fixed?

SLIDE WARMER AT 65 C FOR 2 HOURS

Acid fast bacteria appear what color microscopically?

RED

Acid fast organisms are best stained by which of the following methods?

KINYOUN

In the Ziehl Neelsen staining method, the decolorizer is:

ALCOHOL+HCL

An example of a negative stain is:

INDIA INK STAIN

Which of the following is not a gram negative?

CAMPYLOBACTER

If a patient is taking antimocrobials, which of the following can neutralize the antimicrobials?

THIOL BROTH

From a bronchial washing, organisms are seen on a Gram stain, but no growth occurs aerobically and anaerobically. This is most likely due to:

INHIBITION BY ANTIBIOTIC THERAPY

Which of the following should be used to remove antimicrobials before culturing?

ANTIMICROBIAL REMOVAL DEVICE

Sodium polyanetholsulfonate may be used as an anticoagulant in blood culture because it:

PREVENTS PHAGOCYTOSIS

NEUTRALIZES THE BACTERICIDAL EFFECT OF HUMAN SERUM

What is the most abundant normal flora in throat cultures?

ALPHA-HEMOLYTIC STREPTOCOCCUS

Why must blood plates for throat cultures be incubated aerobically and anaerobically when beta hemolytic strepotococci are suspected?

SOME MAY PRODUCE BETA HEMOLYSIS UNDER AEROBIC CONDITIONS

Nasopharyngeal swabs are recommended for the detection of carriers such as:

HAEMOPHILUS INFLUENZA

NEISSERIA

BORDETELLA PERTUSSIS

Todd-Hewitt broth is recommended for:

CULTURE OF BETA HEMOLYTIC STREPTOCOCCI FOR FLUORESCENCE MICROSCOPY

Phenylethyl alcohol is used in media to:

INHIBIT GRAM NEGATIVE BACTERIA

Why is a first morning urine specimen preferred for urine culture?

SPECIMEN IS MORE CONCENTRATED

What indicates vaginal or urethral contamination of urine?

MANY SQUAMOUS EPITHELIAL CELLS

 

On the pour plate method for colony counts, how does the amount of agar added affect the dilution?

DILUTION IS NOT AFFECTED

Smears of cerebrospinal fluid are prepared from:

CEREBROSPINAL FLUID SEDIMENT

Smears for CSF are usually stained with:

GRAM STAIN AND INDIA INK STAIN

Which of the following tests for bacterial antigen on cultures isolated from CSF is more sensitive and faster?

LATEX AGGLUTINATION TESTS

Staphylococcal protein A coated with antiserum is used in which of the following serological tests on CSF?

COAGGLUTINATION TEST